| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 421 | Mycolicibacterium smegmatis | GTGCTCGGGTCGATCGAAT | TCGAGCGCATCTGCAACT | 59.93 | 59.74 | 259 | 81 |
100.00%
|
100.00%
|
| Primer 422 | Mycolicibacterium smegmatis | CCGTGACAGTGCTCAAGGT | TCGAGCGCATCTGCAACT | 59.93 | 59.74 | 237 | 81 |
100.00%
|
100.00%
|
| Primer 423 | Mycolicibacterium smegmatis | TCTGACCGCAGCGATGAA | TACTCGTCGATCACGCGTG | 59.03 | 59.94 | 243 | 81 |
100.00%
|
100.00%
|
| Primer 424 | Mycolicibacterium smegmatis | TTCGGGTTCGGCTTCGAT | TCGACCAGCAGTTGCAGT | 59.02 | 59.18 | 242 | 81 |
100.00%
|
100.00%
|
| Primer 425 | Mycolicibacterium smegmatis | TGCGCAAACTTCAGGGGT | TTGATCTGTGCGGTCTCGG | 59.81 | 59.78 | 280 | 81 |
100.00%
|
100.00%
|
| Primer 426 | Mycolicibacterium smegmatis | TCAGAACAAGCGCAGGCA | CGTCGGTAAGGAAACGGCT | 59.89 | 60.08 | 195 | 81 |
100.00%
|
100.00%
|
| Primer 427 | Mycolicibacterium smegmatis | TCAGAACAAGCGCAGGCA | GATGTCGTGGATGGTGGCT | 59.89 | 59.78 | 281 | 81 |
100.00%
|
100.00%
|
| Primer 428 | Mycolicibacterium smegmatis | TGATGGTGTTCGTGGTCGG | TTTCGTGGCGTCTGTGCT | 60.01 | 59.89 | 185 | 81 |
100.00%
|
100.00%
|
| Primer 429 | Mycolicibacterium smegmatis | TGTCCGCATCCAGCTGAT | TTTCGTGGCGTCTGTGCT | 58.69 | 59.89 | 199 | 81 |
100.00%
|
100.00%
|
| Primer 430 | Mycolicibacterium smegmatis | ATGTCCGCATCCAGCTGA | TTTCGTGGCGTCTGTGCT | 58.69 | 59.89 | 200 | 81 |
100.00%
|
100.00%
|